The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 22, 2017
Filed:
Jun. 23, 2015
Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);
Chris Schoenherr, Piermont, NY (US);
John McWhirter, Tarrytown, NY (US);
Corey Momont, New York, NY (US);
Caitlin Montagna, Ossining, NY (US);
Lynn Macdonald, Harrison, NY (US);
Gregg S. Warshaw, Bayside, NY (US);
Jose F. Rojas, Newburgh, NY (US);
Ka-Man Venus Lai, Tarrytown, NY (US);
David M. Valenzuela, Yorktown Heights, NY (US);
Andrew J. Murphy, Croton-on-Hudson, NY (US);
Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);
Abstract
Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.