The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 22, 2017

Filed:

Feb. 20, 2014
Applicants:

Kyoto University, Kyoto, JP;

Nipro Corporation, Osaka, JP;

Inventors:

Norio Nakatsuji, Kyoto, JP;

Yoshihiro Yoshikawa, Osaka, JP;

Masakatsu Takeuchi, Osaka, JP;

Daiki Tateyama, Osaka, JP;

Assignees:

KYOTO UNIVERSITY, Kyoto, JP;

Nipro Corporation, Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 1/12 (2006.01);
U.S. Cl.
CPC ...
C12M 23/58 (2013.01); C12M 23/14 (2013.01); C12M 25/02 (2013.01); C12M 29/00 (2013.01); C12M 29/04 (2013.01); C12M 41/00 (2013.01);
Abstract

A culture system has a culture bag for suspending and culturing pluripotent stem cells in a culture medium; a waste liquid container for storing used culture medium that is connected to the culture bag; a fresh medium container for storing a fresh culture medium that is connected to the culture bag; three-way stopcocks for switching flow channels from the culture bag to the waste liquid container or the fresh culture medium container, etc.; a trap portion for trapping the pluripotent stem cells in the culture medium in the flow toward the waste liquid container side; and a filter portion for subculturing that is formed in fourth and fifth flow channels in parallel to a first flow channel. The first flow channel connects the trap part to the culture bag, and has a mesh by which a cell mass of the pluripotent stem cells can be divided. A pump is used to pump the liquid in the individual flow channels.


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