The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 08, 2017

Filed:

Apr. 15, 2014
Applicant:

Nordic Bioscience A/s, Herlev, DK;

Inventors:

Diana Julie Leeming, Klampenborg, DK;

Mette Juul Nielsen, Kobenhavn S, DK;

Morten Karsdal, Kobenhavn O, DK;

Assignee:

Nordic Bioscience A/S, Herlev, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/68 (2006.01); C07K 16/18 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6878 (2013.01); C07K 16/18 (2013.01); C07K 2317/34 (2013.01); G01N 2333/78 (2013.01); G01N 2800/085 (2013.01); G01N 2800/7052 (2013.01);
Abstract

Provided is a monoclonal antibody specifically reactive with a C-terminal neo-epitope of PIIINP comprised in a C-terminal amino acid sequence CPTGXQNYSP-COOH (SEQ ID NO: 4) in which X is Gly or Pro, and where the monoclonal antibody does not recognize or bind an elongated version of the C-terminal amino acid sequence CPTGXQNYSPQZ-COOH (SEQ ID NO: 5), in which Z is absent or is one or more amino acids of the sequence of collagen type III. Also provided is a method of immunoassay for detecting in a biological sample the C-terminal neo-epitope of PIIINP generated by N-protease cleavage of intact type III procollagen, by contacting the sample with the monoclonal antibody, and determining the amount of binding of the antibody.


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