The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2017
Filed:
May. 05, 2011
Alysson Muotri, La Jolla, CA (US);
Paulo Andre Nobrega Marinho, La Jolla, CA (US);
Stevens Kastrup Rehen, Rio de Janeiro, BR;
Leda Dos Reis Castilho, Rio de Janeiro, BR;
Alysson Muotri, La Jolla, CA (US);
Paulo Andre Nobrega Marinho, La Jolla, CA (US);
Stevens Kastrup Rehen, Rio de Janeiro, BR;
Leda Dos Reis Castilho, Rio de Janeiro, BR;
The Regents of the University of California, Oakland, CA (US);
Universidade Federal Do Rio De Janeiro, Rio de Janeiro, BR;
Abstract
The invention provides a defined low protein culture medium for maintaining cells in an undifferentiated state, the medium comprising: a basal medium, an organic acid from the tricarboxylic acid cycle, nonessential amino acids, a combination of growth factors selected from the group consisting of FGF-2 protein, an IGF-1 protein or insulin, a Transferrin protein, and a TGF beta 1 protein, wherein the medium is essentially feeder-free, essentially xeno-free, essentially free of beta-mercaptoethanol, and essentially free of animal-derived or human-derived proteins.