The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 01, 2017

Filed:

Nov. 08, 2012
Applicant:

Université Laval, Québec, CA;

Inventors:

Michel Piché, Québec, CA;

Harold Dehez, Québec, CA;

Yves De Koninck, Québec, CA;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G06K 9/00 (2006.01); G06T 3/40 (2006.01); G02B 21/00 (2006.01); G02B 21/16 (2006.01); G02B 21/36 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
G06T 3/4053 (2013.01); G01N 21/6458 (2013.01); G02B 21/002 (2013.01); G02B 21/16 (2013.01); G02B 21/367 (2013.01); G02B 2207/113 (2013.01); G06T 2207/20224 (2013.01);
Abstract

A method and a system for obtaining a high-resolution image of a volume of a sample using laser imaging are provided. The method includes a step of probing the volume of the sample with a first excitation beam having an intensity profile of maximum intensity at a center thereof, thereby obtaining a positive image of the volume. The method also includes a step of probing the volume of the sample with a second excitation beam having an intensity profile of minimum intensity at a center thereof and defining a peripheral region of maximum intensity around the center, thereby obtaining a negative image of the volume. The method finally includes a step of subtracting the negative image from the positive image, thereby obtaining the high-resolution image of the volume of the sample. Advantageously, embodiments of the invention can be probe- and fluorescence-independent, and be conveniently retrofitted into existing laser imaging systems.


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