The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 01, 2017

Filed:

Jun. 22, 2016
Applicant:

Max-planck-gesellschaft Zur Foerderung Der Wissenschaften E.v., Munich, DE;

Inventor:

Stefan W. Hell, Goettingen, DE;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); G01B 11/14 (2006.01); G02B 21/00 (2006.01); G02B 21/16 (2006.01); G02B 21/36 (2006.01); G02B 27/58 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6458 (2013.01); G01B 11/14 (2013.01); G02B 21/008 (2013.01); G02B 21/0032 (2013.01); G02B 21/0076 (2013.01); G02B 21/16 (2013.01); G02B 21/367 (2013.01); G02B 27/58 (2013.01); G01N 2201/068 (2013.01); G01N 2201/10 (2013.01);
Abstract

In order to determine the locations of individual fluorescent molecules in a sample, which keep a minimum distance with regard to each other, the individual molecules are excited for emission of fluorescence light by means of excitation light. The fluorescence light is registered for different positions of a zero point of an intensity distribution of the excitation light. The distance between these positions is at least half the minimum distance of the fluorescent molecules. The locations of the fluorescent molecules are derived from the course of the intensity of the fluorescence light over the positions of the zero point of the excitation light.


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