The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 01, 2017

Filed:

May. 30, 2014
Applicant:

Inter American University of Puerto Rico, San Juan, PR (US);

Inventor:

Oscar N. Ruiz, Bellbrook, OH (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/04 (2006.01); C12N 15/70 (2006.01); C02F 3/34 (2006.01); C12N 1/20 (2006.01); C12Q 1/34 (2006.01); G01N 33/52 (2006.01); G01N 33/84 (2006.01); C02F 101/20 (2006.01);
U.S. Cl.
CPC ...
C12N 15/70 (2013.01); C02F 3/341 (2013.01); C12N 1/20 (2013.01); C12Q 1/34 (2013.01); G01N 33/523 (2013.01); G01N 33/84 (2013.01); C02F 2101/20 (2013.01);
Abstract

The invention provides a system of heavy metal sequestration by bacteria. The bacteria expresses the ppk, mt, and/or β-galactosidase (lacZ) genes and can tolerate at least 25 μM mercury, 1,000 μM zinc, 250 μM cadmium, and 3,000 μM Pb. The system allows for facile determination of the presence of heavy metal contaminants in a liquid and the facile collection of the bacteria that has sequestered large amounts of heavy metal. Further provided is a system of gene expression in bacteria that comprises phage and plastid gene expression elements and delivers a particularly high level of protein expression and heavy metal resistance.


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