The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 01, 2017

Filed:

Sep. 06, 2013
Applicants:

The United States of America, As Represented BY the Secretary, Department of Health & Human Services, Washington, DC (US);

The Regents of the University of California, Oakland, CA (US);

Inventors:

Bruce A. Shapiro, Gaithersburg, MD (US);

Kirill A. Afonin, Charlotte, NC (US);

Maria L. Kireeva, Gaithersburg, MD (US);

Mikhail Kashlev, Frederick, MD (US);

Luc Jaeger, Goleta, CA (US);

Wade W. Grabow, Shoreline, WA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 15/111 (2013.01); C12P 19/34 (2013.01); C12N 2310/14 (2013.01); C12N 2310/33 (2013.01); C12N 2310/3519 (2013.01); C12N 2310/51 (2013.01); C12N 2310/52 (2013.01); C12N 2320/32 (2013.01); C12N 2330/50 (2013.01);
Abstract

A method is provided for generating RNA nanoparticles having modified nucleotides and/or having increased nuclease resistance where the RNA nanoparticles are formed cotranscriptionally by T7 RNA polymerase in the presence of manganese ions.


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