The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 01, 2017
Filed:
Jun. 15, 2015
Darryl J. Pappin, Boxborough, MA (US);
John P. Wilson, Huntington, NY (US);
Jonathan J. Ipsaro, Cold Spring Harbor, NY (US);
Darryl J. Pappin, Boxborough, MA (US);
John P. Wilson, Huntington, NY (US);
Jonathan J. Ipsaro, Cold Spring Harbor, NY (US);
Other;
Abstract
The invention relates to protease enzymes (that can be thermostable in some embodiments) with unique cleavage specificity as well as their production, isolation, activation and applications. These enzymes can be engineered for quick production and purification. The peptides produced by the action of these protease enzymes have unique properties for biochemical determination of protein sequence. In particular, using the steps of protease digestion, ionization of the specifically produced peptides and fragmentation of these peptides in a mass spectrometer, the amino acid sequence of the peptide may be read as a ladder from the N-terminus.