The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 01, 2017
Filed:
Mar. 21, 2016
Applicant:
Integrated Micro-chromatography Systems, Columbia, SC (US);
Inventor:
Lim Andrew Lee, Columbia, SC (US);
Assignee:
INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, Irmo, SC (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/24 (2006.01); C12Q 1/34 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2402 (2013.01); C12Q 1/34 (2013.01); C12Y 302/01031 (2013.01);
Abstract
Mutatedsp. RLH1 β-glucuronidase enzymes with enhanced enzymatic activity and thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.