The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 25, 2017
Filed:
Apr. 24, 2008
Satoshi Tanaka, Yokohama, JP;
Yoichi Ishizaki, Yokohama, JP;
Ryo Suenaga, Yokohama, JP;
Yoriko Tokita, Kanagawa, JP;
Masahito Kogure, Kanagawa, JP;
Satoshi Tanaka, Yokohama, JP;
Yoichi Ishizaki, Yokohama, JP;
Ryo Suenaga, Yokohama, JP;
Yoriko Tokita, Kanagawa, JP;
Masahito Kogure, Kanagawa, JP;
Toyo Seikan Group Holdings, LTD., Tokyo, JP;
Abstract
In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps of adjusting the pH of said culture medium to be added to be higher than the pH which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved carbon dioxide of the culture medium to be added to be lower than the pressure which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved oxygen of the culture medium to be added to be higher than the pressure which is optimum for culture of the cells; and mixing the adjusted culture medium and the culture medium in said culture-container to adjust at least any of the pH, the partial pressure of dissolved carbon dioxide and the partial pressure of dissolved oxygen to be optimum for culture of said cells, thereby to culture the cells in the culture container.