The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 25, 2017

Filed:

Jul. 16, 2014
Applicant:

National Health Research Institutes, Miaoli County, TW;

Inventors:

Wenya Huang, Tainan, TW;

Ih-Jen Su, Miaoli County, TW;

Yun-Ping Lee, Tainan, TW;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); C07K 16/08 (2006.01); G01N 33/574 (2006.01); G01N 33/576 (2006.01);
U.S. Cl.
CPC ...
C07K 16/082 (2013.01); G01N 33/5764 (2013.01); G01N 33/57438 (2013.01); C07K 2317/34 (2013.01); G01N 2800/085 (2013.01); G01N 2800/50 (2013.01); G01N 2800/54 (2013.01);
Abstract

A HBS-specific antibody, a LHBS-specific antibody, a WT LHBS-specific antibody, an immunoassay kit comprising the antibodies, and a method of detecting pre-Sdeletion mutant LHBS using the immunoassay kit are disclosed herein. The method comprises incubating a biological sample with a first antibody to captured HBS proteins; detecting the LHBS and WT LHBS bound to the immobilized first antibody, respectively; and calculating the amount of the pre-Sdeletion mutant LHBS protein by subtracting the amount of the WT LHBS protein from that of the LHBS protein. Advantageously, by the method described herein, the amount of the pre-Sdeletion mutant LHBS, a potential high-risk marker for HCC incidence in chronic HBV carriers and recurrence in HCC patients after hepatectomy surgery, in a biological sample may be easily calculated without mutual influence between the WT and pre-S mutant LHBS while reducing the labor-intensive process for cloning each gene product before analysis.


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