The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 18, 2017

Filed:

Mar. 14, 2013
Applicant:

Stemcell Technologies Inc., Vancouver, CA;

Inventors:

Alexandra A. Blak, Frechen, DE;

Sharon A. Louis, Vancouver, CA;

Michael John Riedel, Vancouver, CA;

Assignee:

Stemcell Technologies Inc., Vancouver, British Columbia, CA;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12N 5/0735 (2010.01); C12N 5/0797 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0608 (2013.01); C12N 5/0606 (2013.01); C12N 5/0623 (2013.01); C12N 2500/60 (2013.01); C12N 2500/90 (2013.01); C12N 2500/92 (2013.01); C12N 2500/98 (2013.01); C12N 2502/13 (2013.01); C12N 2506/02 (2013.01); C12N 2509/00 (2013.01); C12N 2533/32 (2013.01); C12N 2533/90 (2013.01);
Abstract

The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.


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