The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 11, 2017

Filed:

Dec. 03, 2012
Applicant:

Bio Rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Roger Walker, Benicia, CA (US);

Yabin Lu, Pleasanton, CA (US);

Urvee Desai, San Ramon, CA (US);

Daming Shan, Shoreline, WA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); C12N 9/10 (2006.01); C07K 14/415 (2006.01); G01N 33/564 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6893 (2013.01); C07K 14/415 (2013.01); C12N 9/1044 (2013.01); C12Y 203/02013 (2013.01); G01N 33/564 (2013.01); C07K 2319/21 (2013.01); C07K 2319/23 (2013.01); G01N 2800/06 (2013.01);
Abstract

The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.


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