The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 11, 2017
Filed:
Nov. 20, 2013
Applicant:
Centre National DE LA Recherche Scientifique, Paris, FR;
Inventors:
Jean-Marc Malinge, Olivet, FR;
Patrick Midoux, Saint Denis de l'Hotel, FR;
Thomas Thibault, Villedieu sur Indre, FR;
Chantal Pichon, Saint Denis de l'Hotel, FR;
Assignee:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris, FR;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 15/64 (2006.01); C12N 15/113 (2010.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/10 (2013.01); C12N 15/113 (2013.01); C12N 15/64 (2013.01); A61K 48/00 (2013.01);
Abstract
A method for the in vitro production of DNA minicircles includes steps of: a) providing nicked double-stranded oligodeoxynucleotides bunt-ended substrates having at least one phosphorylated 5' end, b) performing a ligase-mediated circularization on a reaction mixture including the nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.