The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 04, 2017
Filed:
Mar. 17, 2009
Anne Douwe DE Boer, Dreumel, NL;
Michaël Johannes Marcus Ebskamp, Nieuwegein, NL;
Simon Albertus Langeveld, Bennebroek, NL;
Ivo Laros, Renkum, NL;
Miranda Debora Van DE Rhee, Rhenen, NL;
Anne Douwe De Boer, Dreumel, NL;
Michaël Johannes Marcus Ebskamp, Nieuwegein, NL;
Simon Albertus Langeveld, Bennebroek, NL;
Ivo Laros, Renkum, NL;
Miranda Debora Van De Rhee, Rhenen, NL;
STICHTING GENETWISTER IP, Wageningen, NL;
Abstract
The invention relates to a method for analyzing a genomic region of an organism, comprising four major parts. The first part involves the isolation of mRNA from a selected organism that is used for the preparation of small single stranded DNA fragments with one adaptor containing an affinity label. These DNA fragments are used in part three. In the second part, genomic DNA from the same or a related organism is isolated. This genomic DNA is fragmented and ligated to adaptor molecules. In the third part, these genomic fragments are hybridized with single stranded DNA fragments from part one, and the hybrids formed in this process are used for synthesis of DNA fragments. These fragments will be used in part four which involves sequencing of these fragments using one of the available high throughput sequencing methods.