The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 04, 2017

Filed:

Dec. 10, 2013
Applicant:

Academia Sinica, Taipei, TW;

Inventors:

Che-Kun James Shen, Taipei, TW;

Chun-Chang Chen, Changhua County, TW;

Assignee:

ACADEMIA SINICA, Taipei, TW;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/48 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/48 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6897 (2013.01); G01N 2333/91011 (2013.01); G01N 2500/04 (2013.01);
Abstract

Methods for identifying a test agent as a modulator of the active DNA demethylation activity of a DNA methyltransferase are disclosed. The method comprises: a) providing a methylated DNA; b) providing the DNA methyltransferase; c) allowing the methylated DNA to react with the DNA methyltransferase for a sufficient time to perform a demethylation reaction and generate a demethylated DNA product in the presence or absence of a test agent; d) analyzing the extent of demethylation; and d) comparing the extents of the demethylation in the presence and absence of the test agent, and thereby identify the test agent as a modulator of the DNA demethylation activity of the DNA methyltransferase.


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