The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 04, 2017
Filed:
Mar. 06, 2014
Henkel Ag & Co. Kgaa, Duesseldorf, DE;
Petra Siegert, Haan, DE;
Stefan Evers, Haan, DE;
Marion Merkel, Cologne, DE;
Nina Mussmann, Willich, DE;
Hendrik Hellmuth, Duesseldorf, DE;
Timothy O'Connell, Duesseldorf, DE;
Karl-Heinz Maurer, Erkrath, DE;
Ulrich Schwaneberg, Kelmis-Hergenrath, BE;
Felix Jakob, Erkelenz, DE;
Ronny Martinez, Aachen, DE;
Brian Laufs, Juechen, DE;
Ayhan Aydemir, Duesseldorf, DE;
Thomas Weber, Dormagen, DE;
Henkel AG & Co. KGaA, Duesseldorf, DE;
Abstract
The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.