The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 04, 2017

Filed:

Jul. 10, 2014
Applicant:

Tsinghua University, Beijing, CN;

Inventors:

Rong Zhu, Beijing, CN;

Xiaoliang Guo, Beijing, CN;

Assignee:

Tsinghua University, Beijing, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12N 13/00 (2006.01); G01N 33/50 (2006.01); G01N 33/483 (2006.01);
U.S. Cl.
CPC ...
C12N 13/00 (2013.01); G01N 33/4836 (2013.01); G01N 33/5005 (2013.01); B01J 2219/00533 (2013.01); B01J 2219/00653 (2013.01); B01J 2219/00743 (2013.01);
Abstract

The present invention relates to single cell array micro-chips and fabrication, electrical measurement and electroporation method thereof. The single cell array microchip comprises a substrate (), a plurality of positioning electrodes () formed in an array, a plurality of measuring electrode-pairs () formed in an array, and a micro sample pool (). The invention integrates cell array positioning with electrical measurement and electroporation for living cells, which is characteristic of label-free and noninvasive methods to manipulate, position particles/cells as well as further measure their electrical parameters. Therefore, single-cell-array positioning and multi-mode in-situ real-time measurement can be realized for intensive analysis. Since the positioned cells are immobile, the precision of the electrical measurement of cells is effectively improved, so is the efficiency of electroporation with lower cell mortality rate.


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