The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 20, 2017

Filed:

Sep. 27, 2011
Applicants:

Lalitha Kodandapani, San Diego, CA (US);

Louis H. Bookbinder, San Diego, CA (US);

Gregory I. Frost, Del Mar, CA (US);

Philip Lee Sheridan, San Diego, CA (US);

Harold Michael Shepard, San Diego, CA (US);

GE Wei, San Diego, CA (US);

Lei Huang, San Diego, CA (US);

Inventors:

Lalitha Kodandapani, San Diego, CA (US);

Louis H. Bookbinder, San Diego, CA (US);

Gregory I. Frost, Del Mar, CA (US);

Philip Lee Sheridan, San Diego, CA (US);

Harold Michael Shepard, San Diego, CA (US);

Ge Wei, San Diego, CA (US);

Lei Huang, San Diego, CA (US);

Assignee:

Halozyme, Inc., San Diego, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); C07K 16/28 (2006.01); C07K 16/30 (2006.01); C07K 16/44 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5011 (2013.01); C07K 16/2863 (2013.01); C07K 16/30 (2013.01); C07K 16/44 (2013.01); G01N 33/5014 (2013.01); G01N 33/5088 (2013.01); G01N 33/53 (2013.01); C07K 2317/34 (2013.01); G01N 2333/71 (2013.01);
Abstract

Methods for evolving or selecting or producing therapeutic proteins that exhibit reduced adverse side-effects and the resulting proteins are provided. For example, provided herein is an in vitro assay to identify conditionally active therapeutic proteins that exhibit better activity within one in vivo environment compared to another in vivo environment. The methods include the steps of a) testing the activity of a protein under conditions in which normal or increased activity is desired; b) testing the activity of the protein under conditions in which reduced activity compared to normal is desired; and c) comparing the activity in a) with b) and selecting/identifying a protein that has greater activity in a) compared to b). The selected/identified protein is a conditionally active protein.


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