The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 20, 2017
Filed:
Dec. 28, 2011
Applicant:
Jay M. Short, Del Mar, CA (US);
Inventor:
Jay M. Short, Del Mar, CA (US);
Assignee:
BioAlta, LLC, San Diego, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/46 (2006.01); C07K 16/24 (2006.01);
U.S. Cl.
CPC ...
C07K 16/461 (2013.01); C07K 16/248 (2013.01); C07K 16/464 (2013.01); C07K 2317/92 (2013.01);
Abstract
The disclosure provides a method for generation of humanized full length antibodies in mammalian cells. A library of humanized variants is provided with high, validated human framework diversity without requiring back-mutations to retain original affinity. Synthetic CDR encoding fragment libraries derived from a template antibody are ligated to human framework region encoding fragments from a human framework pool limited only to germline sequences from a functionally expressed anti-bodies. The vector comprises a nucleic acid sequence encoding HC framework region 4. No CDR grafting or phage display is required.