The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 13, 2017

Filed:

Mar. 09, 2009
Applicants:

Michael G. Pollack, Durham, NC (US);

Ramakrishna Sista, Morrisville, NC (US);

Zhishan Hua, Cary, NC (US);

Vamsee K. Pamula, Durham, NC (US);

Arjun Sudarsan, Cary, NC (US);

Vijay Srinivasan, Durham, NC (US);

Prasanna Thwar, Morrisville, NC (US);

Alexander Shenderov, Raleigh, NC (US);

Dwayne Allen, Durham, NC (US);

Inventors:

Michael G. Pollack, Durham, NC (US);

Ramakrishna Sista, Morrisville, NC (US);

Zhishan Hua, Cary, NC (US);

Vamsee K. Pamula, Durham, NC (US);

Arjun Sudarsan, Cary, NC (US);

Vijay Srinivasan, Durham, NC (US);

Prasanna Thwar, Morrisville, NC (US);

Alexander Shenderov, Raleigh, NC (US);

Dwayne Allen, Durham, NC (US);

Assignee:

ADVANCED LIQUID LOGIC, INC., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01L 3/02 (2006.01); B01F 13/00 (2006.01); B01F 13/08 (2006.01); B03C 1/01 (2006.01); B03C 1/28 (2006.01);
U.S. Cl.
CPC ...
B01L 3/0282 (2013.01); B01F 13/002 (2013.01); B01F 13/0023 (2013.01); B01F 13/0071 (2013.01); B01F 13/0076 (2013.01); B01F 13/0818 (2013.01); B01L 3/502753 (2013.01); B03C 1/01 (2013.01); B03C 1/284 (2013.01); B03C 1/286 (2013.01); B01L 2200/0621 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/141 (2013.01); B01L 2300/0832 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/043 (2013.01); B03C 2201/18 (2013.01); B03C 2201/26 (2013.01); B03C 2201/28 (2013.01); Y10T 436/25 (2015.01);
Abstract

Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.


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