The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 06, 2017

Filed:

Aug. 27, 2008
Applicants:

Kwang Lae Hoe, Daejeon, KR;

Dong Uk Kim, Daejeon, KR;

MI Sun Won, Daejeon, KR;

Hyang Sook Yoo, Daejeon, KR;

Dong Sup Kim, Daejeon, KR;

Han OH Park, Daejeon, KR;

Kyung Sook Chung, Daejeon, KR;

Young Joo Jang, Seoul, KR;

MI Young Nam, Daejeon, KR;

Sang JO Han, Daejeon, KR;

Shin Jung Choi, Daejeon, KR;

Seung Tae Baek, Daejeon, KR;

Hyong Bai Kim, Seoul, KR;

Kyung Sun Heo, Daejeon, KR;

Hye MI Lee, Daejeon, KR;

Min Ho Lee, Daejeon, KR;

JO Young Park, Chungcheongnam-do, KR;

Inventors:

Kwang Lae Hoe, Daejeon, KR;

Dong Uk Kim, Daejeon, KR;

Mi Sun Won, Daejeon, KR;

Hyang Sook Yoo, Daejeon, KR;

Dong Sup Kim, Daejeon, KR;

Han Oh Park, Daejeon, KR;

Kyung Sook Chung, Daejeon, KR;

Young Joo Jang, Seoul, KR;

Mi Young Nam, Daejeon, KR;

Sang Jo Han, Daejeon, KR;

Shin Jung Choi, Daejeon, KR;

Seung Tae Baek, Daejeon, KR;

Hyong Bai Kim, Seoul, KR;

Kyung Sun Heo, Daejeon, KR;

Hye Mi Lee, Daejeon, KR;

Min Ho Lee, Daejeon, KR;

Jo Young Park, Chungcheongnam-do, KR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C40B 50/00 (2006.01); C40B 40/02 (2006.01); C40B 30/06 (2006.01); C12N 1/00 (2006.01); C12N 15/10 (2006.01); C40B 30/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1034 (2013.01); C40B 30/00 (2013.01); C40B 40/02 (2013.01); C40B 50/00 (2013.01); C12N 1/00 (2013.01);
Abstract

A method comprising transformingwith a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion. Also provided are gene-targeted hetero2ygous deletionmutants prepared by the method, and a library of gene-targeted heterozygous deletionmutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.


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