The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 06, 2017

Filed:

Apr. 25, 2014
Applicant:

The Trustees of the University of Pennsylvania, Philadelphia, PA (US);

Inventors:

Renyu Liu, Media, PA (US);

Jeffery G Saven, Philadelphia, PA (US);

Jose Manuel Perez-Aguilar, New York, NY (US);

Felipe Matsunaga, Olmsted Township, OH (US);

Jin Xi, Media, PA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/00 (2006.01); C07K 14/705 (2006.01); G01N 33/74 (2006.01); G01N 33/566 (2006.01);
U.S. Cl.
CPC ...
C07K 14/705 (2013.01); A61K 38/00 (2013.01); G01N 33/566 (2013.01); G01N 33/74 (2013.01); C07K 2319/21 (2013.01); C07K 2319/42 (2013.01); G01N 2500/04 (2013.01);
Abstract

Described herein are recombinant integral membrane proteins having multiple transmembrane domains that have been engineered to be less hydrophobic, through alteration of the amino acid sequence of the native protein, but retain the ability to bind their natural ligand. The decreased hydrophobicity of the described proteins makes them more water soluble than the native protein, which allows the described proteins to be expressed in bacteria in large quantities and isolated in the absence of membranes, all while retaining the ability to interact with known ligands.


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