The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 30, 2017

Filed:

Sep. 12, 2008
Applicants:

Musa Mhlanga, Paris, FR;

Jost Enninga, Paris, FR;

Philippe Sansonetti, Paris, FR;

Ulf Nehrbass, Paris, FR;

Inventors:

Musa Mhlanga, Paris, FR;

Jost Enninga, Paris, FR;

Philippe Sansonetti, Paris, FR;

Ulf Nehrbass, Paris, FR;

Assignee:

INSTITUT PASTEUR, Paris, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6897 (2013.01); C12Q 1/6897 (2013.01); C12Q 2563/179 (2013.01); C12Q 2525/301 (2013.01); C12Q 2565/1015 (2013.01);
Abstract

The invention relates to a double-stranded polynucleotide comprising on its positive strand considered from its 5' end to its 3′ end, (i) a promoter of a gene of interest or several promoters of various genes of interest selected among genes which are endogenous to a determined cell, and, (ii) one or several barcode(s) wherein each barcode contains at least one barcode unit formed of at least one, especially of multiple, recognition binding sites each binding site being composed of a nucleotide sequence, and wherein each of said barcode(s) is under the control of at least one of said promoter(s) for transcription. It further concerns use of said polynucleotide to monitor gene expression patterns in living cells, especially in single cells, with a rapid and high spatio-temporal resolution.


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