The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 30, 2017

Filed:

Apr. 05, 2012
Applicants:

Lukas Maksym, Cologne, DE;

Ramona Knab, Neuss, DE;

Stefan Evers, Mettmann, DE;

Karl-heinz Maurer, Erkrath, DE;

Johannes Bongaerts, Dormagen, DE;

Inventors:

Lukas Maksym, Cologne, DE;

Ramona Knab, Neuss, DE;

Stefan Evers, Mettmann, DE;

Karl-Heinz Maurer, Erkrath, DE;

Johannes Bongaerts, Dormagen, DE;

Assignee:

BASF SE, Ludwigshafen, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/87 (2006.01); C12P 21/00 (2006.01); C12N 9/48 (2006.01); C12N 15/67 (2006.01); C12P 21/02 (2006.01); C12N 9/54 (2006.01);
U.S. Cl.
CPC ...
C12N 15/87 (2013.01); C12N 9/48 (2013.01); C12N 9/54 (2013.01); C12N 15/67 (2013.01); C12P 21/00 (2013.01); C12P 21/02 (2013.01);
Abstract

The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.


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