The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 23, 2017
Filed:
May. 09, 2014
The Regents of the University of California, Oakland, CA (US);
Zuzanna S. Siwy, Irvine, CA (US);
Kenneth J. Shea, Irvine, CA (US);
Ken Healy, Auburn, AL (US);
Laura Michele Innes, Cerritos, CA (US);
Matthew Schiel, Irvine, CA (US);
Matthew Pevarnik, Irvine, CA (US);
The Regents of the University of California, Oakland, CA (US);
Abstract
A method for multiplex characterization of individual particles by their size, shape, mechanical properties (deformability), and chemical affinity to recognition agents. The analysis can be performed from concentrated solutions. The method detects transient sticking of particles in the pore and points to its location along a pore axis. If a pore is decorated with a recognition agent for an analyte present in a solution, it is possible to distinguish specific binding at the place of the recognition agent, and non-specific adsorption of the analyte. The method confirms whether any individual particle or hydrogel completely translocates the pore and allows unambiguous detection and characterization of multiple particles or hydrogels in the pore, which would previously corrupt the results, so that higher analyte concentrations can be used for faster analysis. High aspect ratio of the pores (ratio of pore length and diameter) together with the pattern of ion current pulses also allow passage of the same particle or cell multiple times without letting the cell exit the pore.