The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 23, 2017
Filed:
Dec. 03, 2010
Fredrik Roos, Loveland, CO (US);
Henrik Johansson, Loveland, CO (US);
Magnus Isaksson, Loveland, CO (US);
Mats Nilsson, Loveland, CO (US);
Olle (Olof) Ericsson, Uppsala, SE;
Simon Fredriksson, Loveland, CO (US);
Fredrik Roos, Loveland, CO (US);
Henrik Johansson, Loveland, CO (US);
Magnus Isaksson, Loveland, CO (US);
Mats Nilsson, Loveland, CO (US);
Olle (Olof) Ericsson, Uppsala, SE;
Simon Fredriksson, Loveland, CO (US);
Agilent Technologies, Inc., Santa Clara, CA (US);
Abstract
A method for amplifying a target nucleic acid is disclosed, which includes: (a) fragmenting a nucleic acid sample to create a target fragment comprising a target nucleic acid and two probe-complementary portions; (b) contacting said fragmented nucleic acid sample with a probe comprising two target fragment-complementary portions complementary to the probe-complementary portions of the target fragment; (c) rendering the fragmented nucleic acid sample single-stranded; (d) allowing the probe-complementary portions to hybridise with the target-fragment complementary portions; (e) if the probe in step (b) is not immobilised, immobilising the probe-target fragment hybrid on a solid phase via immobilisation moiety; (f) separating non-immobilised nucleic acid fragments from the solid phase; (g) contacting the solid phase with a ligase to ligate ligatable 5' and 3′ ends of the target fragment whereby the target fragment is circularized; and (h) amplifying said circularized target fragment.