The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 23, 2017

Filed:

Mar. 04, 2011
Applicants:

H. Randall Bell, Arlington, VA (US);

Joan M Bienvenue, Fredericksburg, VA (US);

John W Pettit, Derwood, MD (US);

James P Landers, Charlottesville, VA (US);

Jessica V Norris, Palmyra, VA (US);

Orion N Scott, Charlottesville, VA (US);

Daniel J Marchiarullo, North Haledon, NJ (US);

Daniel C Leslie, Brookline, MA (US);

Inventors:

H. Randall Bell, Arlington, VA (US);

Joan M Bienvenue, Fredericksburg, VA (US);

John W Pettit, Derwood, MD (US);

James P Landers, Charlottesville, VA (US);

Jessica V Norris, Palmyra, VA (US);

Orion N Scott, Charlottesville, VA (US);

Daniel J Marchiarullo, North Haledon, NJ (US);

Daniel C Leslie, Brookline, MA (US);

Assignees:

LEIDOS INNOVATIONS TECHNOLOGY, INC., Gaithersburg, MD (US);

ZyGEM CORPORATION LTD., Hamilton, NZ;

UNIVERSITY OF VIRGINIA PATENT FOUNDATION, Charlottesville, VA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 27/447 (2006.01); G01N 29/32 (2006.01); G01N 21/41 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502753 (2013.01); B01L 7/525 (2013.01); G01N 27/44791 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0816 (2013.01); B01L 2400/0421 (2013.01); G01N 29/32 (2013.01); G01N 2021/4186 (2013.01);
Abstract

Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.


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