The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 16, 2017
Filed:
Jun. 27, 2011
Applicants:
Thomas Froehlich, Bichl, DE;
Tobias Heckel, Basel, CH;
Inventors:
Thomas Froehlich, Bichl, DE;
Tobias Heckel, Basel, CH;
Assignee:
Roche Molecular Systems, Inc., Pleasanton, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1075 (2013.01); C12N 15/10 (2013.01); C12N 15/1096 (2013.01);
Abstract
Disclosed is a process for clonal pre-amplification of a nucleic acid involving the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3' ends and 5′ ends of the nucleic acid molecules (c) preparing a water in oil emulsion wherein the majority of water droplets comprises one or none member of the plurality of different nucleic acid molecules (d) clonally amplifying the plurality of different nucleic acid molecules. In particular, the different nucleic acid molecules are mRNA molecules.