The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 16, 2017

Filed:

Feb. 26, 2013
Applicant:

Yamasa Corporation, Choshi-shi, CH;

Inventors:

Kaori Tanabe, Choshi, JP;

Kazuya Ishige, Choshi, JP;

Assignee:

Yamasa Corporation, Choshi-shi, Chiba, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12P 19/36 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1241 (2013.01); C12P 19/36 (2013.01); C12Y 207/07065 (2013.01); C07K 2319/00 (2013.01);
Abstract

A practical method for enzymatically synthesizing c-di-GMP with excellent productivity is provided. A diguanylate cyclase having physical and chemical characteristics (A) to (F): (A) catalytic action on reaction '2 GTP→c-di-GMP'; (B) a molecular weight of 19800±2000; (C) an optimum pH of 7.3 to 9.4; (D) an optimum temperature of 35 to 60° C.; (E) thermal stability as the remaining activity of 90% or higher after heated for 60 minutes under conditions of 50° C. and pH7.8; and (F) the presence of GGDEF (SEQ ID NO:26) domain and the lack of amino acid sequence KXXD (SEQ ID NO:23) in the i-site.


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