The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 09, 2017

Filed:

Oct. 27, 2010
Applicants:

Jan Johansson, Stockholm, SE;

Anna Rising, Uppsala, SE;

MY Hedhammar, Stockholm, SE;

Kerstin Nordling, Knivsta, SE;

Inventors:

Jan Johansson, Stockholm, SE;

Anna Rising, Uppsala, SE;

My Hedhammar, Stockholm, SE;

Kerstin Nordling, Knivsta, SE;

Assignee:

SPIBER TECHNOLOGIES AB, Uppsala, SE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/435 (2006.01); C12N 15/62 (2006.01);
U.S. Cl.
CPC ...
C07K 14/43518 (2013.01); C12N 15/62 (2013.01);
Abstract

A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.


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