The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 09, 2017

Filed:

Apr. 04, 2012
Applicants:

Marc Unger, South San Francisco, CA (US);

Ian D. Manger, San Francisco, CA (US);

Michael Lucero, South San Francisco, CA (US);

Yong Yi, San Francisco, CA (US);

Emily Miyashita-lin, San Francisco, CA (US);

Anja Wienecke, Burlingame, CA (US);

Geoffrey Facer, San Francisco, CA (US);

Inventors:

Marc Unger, South San Francisco, CA (US);

Ian D. Manger, San Francisco, CA (US);

Michael Lucero, South San Francisco, CA (US);

Yong Yi, San Francisco, CA (US);

Emily Miyashita-Lin, San Francisco, CA (US);

Anja Wienecke, Burlingame, CA (US);

Geoffrey Facer, San Francisco, CA (US);

Assignee:

Fluidigm Corporation, South San Francisco, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12Q 1/68 (2006.01); G01N 35/00 (2006.01); B01F 13/00 (2006.01); C40B 60/14 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502707 (2013.01); B01L 3/50851 (2013.01); B01L 3/502715 (2013.01); C12Q 1/6844 (2013.01); G01N 35/00029 (2013.01); B01F 13/0059 (2013.01); B01J 2219/0059 (2013.01); B01J 2219/0072 (2013.01); B01J 2219/00337 (2013.01); B01J 2219/00353 (2013.01); B01J 2219/00403 (2013.01); B01J 2219/00495 (2013.01); B01J 2219/00585 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/123 (2013.01); B01L 2400/06 (2013.01); C40B 60/14 (2013.01); G01N 35/0098 (2013.01); G01N 2035/00366 (2013.01); Y10T 137/2076 (2015.04); Y10T 137/2496 (2015.04); Y10T 137/86292 (2015.04); Y10T 137/87249 (2015.04); Y10T 436/2575 (2015.01);
Abstract

A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.


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