The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 09, 2017

Filed:

Apr. 25, 2014
Applicants:

California Institute of Technology, Pasadena, CA (US);

The Regents of the University of California, Oakland, CA (US);

Inventors:

Carl L. Hansen, Pasadena, CA (US);

Stephen R. Quake, Stanford, CA (US);

James M. Berger, Kensington, CA (US);

Assignee:

Fluidigm Corporation, South San Francisco, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C30B 7/14 (2006.01); B01F 5/00 (2006.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01); F04B 43/04 (2006.01); F16K 99/00 (2006.01); B81B 1/00 (2006.01); B01F 13/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
B01F 5/00 (2013.01); B01F 13/0093 (2013.01); B01L 3/502738 (2013.01); B01L 3/502746 (2013.01); B01L 3/502769 (2013.01); B01L 9/527 (2013.01); B81B 1/00 (2013.01); F04B 43/043 (2013.01); F16K 99/0001 (2013.01); F16K 99/0015 (2013.01); F16K 99/0017 (2013.01); F16K 99/0059 (2013.01); B01J 2219/005 (2013.01); B01J 2219/0061 (2013.01); B01J 2219/00355 (2013.01); B01J 2219/00378 (2013.01); B01J 2219/00396 (2013.01); B01J 2219/00398 (2013.01); B01J 2219/00439 (2013.01); B01J 2219/00527 (2013.01); B01J 2219/00605 (2013.01); B01J 2219/00612 (2013.01); B01J 2219/00619 (2013.01); B01J 2219/00621 (2013.01); B01J 2219/00635 (2013.01); B01J 2219/00637 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00707 (2013.01); B01J 2219/00722 (2013.01); B01J 2219/00725 (2013.01); B01L 7/54 (2013.01); B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0605 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/123 (2013.01); B01L 2300/14 (2013.01); B01L 2300/18 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/0688 (2013.01); F16K 2099/008 (2013.01); F16K 2099/0074 (2013.01); F16K 2099/0078 (2013.01); F16K 2099/0084 (2013.01); F16K 2099/0086 (2013.01); Y10T 117/1004 (2015.01); Y10T 117/1008 (2015.01); Y10T 117/1012 (2015.01); Y10T 137/0318 (2015.04); Y10T 137/0324 (2015.04); Y10T 137/2224 (2015.04); Y10T 436/25 (2015.01);
Abstract

A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.


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