The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 02, 2017
Filed:
Dec. 14, 2009
Applicant:
Daniel Klein-marcuschamer, San Francisco, CA (US);
Inventor:
Daniel Klein-Marcuschamer, San Francisco, CA (US);
Assignee:
GreenLight Biosciences, Inc., Medford, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/52 (2006.01); C12N 9/22 (2006.01); C12P 7/42 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 9/22 (2013.01); C12P 7/42 (2013.01); Y02P 20/52 (2015.11);
Abstract
The invention pertains to a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest. mRNAs that code for interferase-resistant products are overexpressed in the background of a metabolically-frozen cell. Enzymes that compete for a substrate or product of the pathway of interest may be altered such that the enzyme is sensitive to a site-specific protease, which protease is inducible in the host cell.