The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 02, 2017

Filed:

Jan. 13, 2014
Applicant:

Pbs Biotech, Inc., Camarillo, CA (US);

Inventors:

Chanyong Brian Lee, Camarillo, CA (US);

Yasunori Hashimura, Camarillo, CA (US);

Assignee:

PBS Biotech, Inc., Camarillo, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12M 3/00 (2006.01); C12M 1/12 (2006.01); C12M 1/06 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01);
U.S. Cl.
CPC ...
C12M 25/16 (2013.01); C12M 27/06 (2013.01); C12M 29/04 (2013.01); C12M 41/00 (2013.01); Y10T 137/0318 (2015.04);
Abstract

A system and method for compartmentalizing micro-carriers in a bioreactor includes a container configured to store micro-carriers and a vessel configured to contain a fluid so as to prevent the micro-carriers from entering the vessel during sterilization, shipping, storage, or other pre-use handling of the system. One or more addition lines connect the container and the vessel such that the vessel is in fluid communication with the vessel. The one or more addition lines are contactable by at least one blocking element configured to reversibly block fluid flow between the container and the vessel. The container, addition lines, and vessel are configured to allow the micro-carriers to be injected into the vessel at any point during a cell culture run. The vessel may also include a rotatable wheel, a harvest port configured to allow for the removal of the micro-carriers from the vessel, and a media removal port comprising a retention screen for removing spent medium.


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