The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 25, 2017
Filed:
Sep. 28, 2012
Grasslanz Technology Limited, Palmerston North, NZ;
Richard David Johnson, Palmerston North, NZ;
Christine Rosalie Voisey, Palmerston North, NZ;
GRASSLANZ TECHNOLOGY LIMITED, Hamilton, NZ;
Abstract
The invention provides a method and compositions for detecting the presence of viable endophyte in a plant, the method comprising germinating seed and excising a young leaf, optionally preparing an extract from the young leaf, and performing antibody, nucleic acid, or preferably PCR-based methods to detect the viable endophyte, wherein at least one primer hybridizes to the intergenic region (IGS) of the ribosomal repeat region of a specific endophyte when used in a PCR-based method. The invention provides a new method for rapid identification of both viable beneficial endophytes and any viable contaminating endophytes, as opposed to any non-viable endophytes, by detecting the presence of endophytes in the first leaf of germinated plants.