The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9630158 B1

PDF
Full Text
Expired
Date of Patent:
Apr. 25, 2017

Filed:

Apr. 12, 2010
Applicant:

Hiroshi Inoue, Rockville, MD (US);

Inventor:

Hiroshi Inoue, Rockville, MD (US);

Assignee:

Canon U.S. Life Sciences, Inc., Rockville, MD (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01F 13/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
B01F 13/0093 (2013.01); B01F 13/0071 (2013.01); B01L 3/5027 (2013.01); B01L 7/52 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01);
Abstract

The present invention relates to systems and methods of performing in-line mixing of assay components and delivery of such mixed components into microfluidic channels. In one aspect, a method of delivering mixed assay components is provided which comprises causing an unmixed primer solution to flow into a first mixing channel, the unmixed primer solution comprising a common reagent and a primer, holding the unmixed primer solution in the first mixing channel for at least a threshold amount of time to allow the unmixed primer solution to transition into a mixed primer solution, causing a buffer to flow into a second mixing channel, the buffer comprising the common reagent but not including a primer, and, after holding the unmixed primer solution in the first mixing channel for at least the threshold amount of time, drawing, from the first mixing channel, the mixed primer solution into a common exit channel.


Find Patent Forward Citations

Inoue, Hiroshi. (2017). Method of delivering pcr solution to microfluidic pcr chamber (U.S. Patent No. 9630158). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9630158

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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