The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 18, 2017

Filed:

Jun. 15, 2012
Applicants:

Alice Y. Ting, Allston, MA (US);

Jeffrey Daniel Martell, Cambridge, MA (US);

Hyun-woo Rhee, Gulhwart, KR;

Peng Zou, Cambridge, MA (US);

Inventors:

Alice Y. Ting, Allston, MA (US);

Jeffrey Daniel Martell, Cambridge, MA (US);

Hyun-Woo Rhee, Gulhwart, KR;

Peng Zou, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/28 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/28 (2013.01); G01N 33/5023 (2013.01); G01N 33/6803 (2013.01); G01N 2570/00 (2013.01);
Abstract

The application discloses methods, materials, and compositions for the labeling of molecules, for example, proteins, in living cells or in subcellular compartments of living cells. In particular, the application relates to proteomic analysis methods; materials and compositions and means based on direct tagging of unknown proteins with tagging enzymes (such as biotin ligase or a peroxidase) within the vicinity of a tagging substrate (such as a tyramide) within living cells, with optional targeting to specific subcellular locations by expression of genetic constructs.


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