The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 18, 2017
Filed:
Jul. 18, 2013
South Dakota Board of Regents, Pierre, SD (US);
Cambridge Enterprise Limited, Cambridgeshire, GB;
Academisch Ziekenhuis Leiden, Leiden, NL;
University College Cork, Cork, IE;
Ying Fang, Pierre, SD (US);
Eric John Snijder, Leiden, NL;
Andrew E. Firth, Cambridgeshire, GB;
John F. Atkins, Cork, IE;
Emma Elisabeth Treffers, Leiden, NL;
Ali Tas, Leiden, NL;
Yanhua Li, Pierre, SD (US);
South Dakota Board of Regents, Pierre, SD (US);
Cambridge Enterprise Limited, Cambridgeshire, GB;
Academisch Ziekenhuis Leiden, Leiden, NL;
University College Cork, Cork, IE;
Abstract
The invention provides the discovery and characterization of a novel arterivirus protein (nsp2TF), whose expression is dependent on −2 ribosomal frameshifting at a site located in the nsp2 coding region. The coding region for the unique TF domain of nsp2TF overlaps the part of ORF1a that encodes the transmembrane region of nsp2 in arteriviruses, including PRRSV, LDV and SHFV. Mutations affecting the expression of nsp2TF impair PRRSV replication and result in a smaller plaque phenotype. Provided herein are arteriviruses that display reduced translation of nsp2TF and/or altered translation of one or more downstream products, arteriviruses in which nsp2TF function is reduced and/or absent, and vaccines comprising said arteriviruses. Also provided herein are diagnostic methods, methods for identifying compounds that inhibit −2 frameshifting, and gene expression tools for eukaryotic systems utilizing −2 frameshifting.