The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 11, 2017

Filed:

Apr. 21, 2016
Applicant:

Ajinomoto Co., Inc., Chuo-ku, JP;

Inventors:

Takahiro Nonaka, Kawasaki, JP;

Teruhisa Mannen, Kawasaki, JP;

Noriko Tsurui, Kawasaki, JP;

Assignee:

AJINOMOTO CO., INC., Chou-ku, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C07K 1/30 (2006.01); C07K 14/00 (2006.01); C07K 14/815 (2006.01); C07K 14/32 (2006.01); C07K 1/34 (2006.01); C07K 1/36 (2006.01); C07K 14/34 (2006.01); C07K 14/62 (2006.01); C07K 14/635 (2006.01);
U.S. Cl.
CPC ...
C07K 1/30 (2013.01); C07K 1/34 (2013.01); C07K 1/36 (2013.01); C07K 14/00 (2013.01); C07K 14/32 (2013.01); C07K 14/34 (2013.01); C07K 14/62 (2013.01); C07K 14/635 (2013.01); C07K 14/815 (2013.01); C07K 2319/00 (2013.01); C07K 2319/50 (2013.01); C07K 2319/70 (2013.01);
Abstract

A method of purifying a fusion protein, including: (1) adjusting the pH of an aqueous phase, containing a fusion protein which is a fusion of a protein having a self-assembly capability and a tamet protein, and which aqueous phase has a first pH, to a second pH, to obtain a remaining aqueous phase and a solid fraction containing an amount of the fusion protein; (2) separating the solid fraction from the remaining aqueous phase, to obtain a separated solid fraction; and (3) dissolving the separated solid fraction in a solution having a pH of 12 or lower but higher than the second pH by at least 0.1 pH units, wherein the protein having a self-assembly capability is a cell surface protein.


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