The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 2017

Filed:

May. 21, 2014
Applicant:

University of Washington, Seattle, WA (US);

Inventors:

James E. Bruce, Kennewick, WA (US);

Juan Chavez, Seattle, WA (US);

Chad Weisbrod, Seattle, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/00 (2006.01); C07K 2/00 (2006.01); C07K 4/00 (2006.01); C07K 5/00 (2006.01); C07K 14/00 (2006.01); C07K 16/00 (2006.01); C07K 17/00 (2006.01); G01N 33/68 (2006.01); G06F 19/26 (2011.01); G01N 33/569 (2006.01); G01N 33/574 (2006.01); C07K 7/06 (2006.01); C07K 7/08 (2006.01); G06F 19/16 (2011.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); C07K 7/06 (2013.01); C07K 7/08 (2013.01); G01N 33/56911 (2013.01); G01N 33/57484 (2013.01); G06F 19/26 (2013.01); G01N 2333/212 (2013.01); G01N 2500/02 (2013.01); G06F 19/16 (2013.01);
Abstract

Disclosed herein are methods for large-scale, high-throughput identification of protein-protein interactions and the topologies thereof under physiologically relevant conditions. In one aspect, the disclosure provides methods for identifying one or a plurality of interacting peptides within a biological system comprising obtaining a population of proteins cross-linked with a cleavable protein interaction reporter (PIR) cross-linker, cleaving the PIR crosslinker to produce released peptides and cleaved reporter ions, and analyzing the population of released peptides to identify interacting peptides. Also disclosed are methods for identifying candidate drug compounds, as well as methods of data processing and visualization of protein-protein interactions.


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