The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 2017

Filed:

Jun. 14, 2011
Applicants:

Gwenola Sabatte, Eindhoven, NL;

Menno Willem Jose Prins, Eindhoven, NL;

Toon Hendrik Evers, Eindhoven, NL;

Wilhelmina Maria Hardeman, Eindhoven, NL;

Joukje Garrelina Orsel, Eindhoven, NL;

Inventors:

Gwenola Sabatte, Eindhoven, NL;

Menno Willem Jose Prins, Eindhoven, NL;

Toon Hendrik Evers, Eindhoven, NL;

Wilhelmina Maria Hardeman, Eindhoven, NL;

Joukje Garrelina Orsel, Eindhoven, NL;

Assignee:

Koninklijke Philips N.V., Eindhoven, NL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5306 (2013.01); G01N 33/54306 (2013.01); G01N 33/54333 (2013.01);
Abstract

The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.


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