The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 04, 2017
Filed:
Jul. 24, 2013
Luminex Corporation, Austin, TX (US);
David J. Marshall, Madison, WI (US);
James R. Prudent, Madison, WI (US);
Christopher B. Sherrill, Madison, WI (US);
Gideon Shapiro, Alachua, FL (US);
Jennifer K. Grenier, Madison, WI (US);
Craig S. Richmond, Madison, WI (US);
Simona Jurczyk, Gainesville, FL (US);
Jerod L. Ptacin, Beloit, WI (US);
LUMINEX CORPORATION, Austin, TX (US);
Abstract
Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.