The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 2017

Filed:

Mar. 15, 2013
Applicant:

Butamax(tm) Advanced Biofuels Llc, Wilmington, DE (US);

Inventors:

Dennis Flint, Newark, DE (US);

Brian James Paul, Wilmington, DE (US);

Rick W. Ye, Hockessin, DE (US);

Assignee:

Butamax Advanced Biofuels LLC, Wilmington, DE (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/81 (2006.01); C12N 9/88 (2006.01); C07K 14/395 (2006.01); C12P 7/16 (2006.01); C12P 7/40 (2006.01); C12P 13/02 (2006.01); C12P 13/04 (2006.01); C12Q 1/527 (2006.01); G01N 33/573 (2006.01);
U.S. Cl.
CPC ...
C12N 15/81 (2013.01); C07K 14/395 (2013.01); C12N 9/88 (2013.01); C12P 7/16 (2013.01); C12P 7/40 (2013.01); C12P 13/02 (2013.01); C12P 13/04 (2013.01); C12Q 1/527 (2013.01); C12Y 402/01009 (2013.01); G01N 33/573 (2013.01); Y02E 50/10 (2013.01); Y02P 20/52 (2015.11);
Abstract

The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.


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