The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 28, 2017

Filed:

Feb. 11, 2013
Applicants:

The Children's Medical Center Corporation, Boston, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Elizabeth M. O'Day, Braintree, MA (US);

Judy Lieberman, Brookline, MA (US);

Gerhard Wagner, Chestnut Hill, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); G01N 24/08 (2006.01); G01R 33/46 (2006.01); G01R 33/465 (2006.01); A61B 5/055 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5011 (2013.01); G01N 24/08 (2013.01); G01N 33/5005 (2013.01); G01N 33/5038 (2013.01); G01R 33/465 (2013.01); G01R 33/4633 (2013.01); A61B 5/055 (2013.01); G01N 2458/15 (2013.01); G01N 2500/10 (2013.01); G01N 2570/00 (2013.01);
Abstract

Methods that enable one to specifically measure the metabolic product of a particular molecule in relatively few cells, e.g. primary cells, are described. The methods involve optionally preloading cells with labeled substrate (e.g. labeled byC,N, orP). The methods allow for easy identification of metabolites that are differentially generated in cells of different phenotypes. The new methods for unbiased multi-dimensional NMR screening and rapid and efficient analysis of the NMR screening identify differentially expressed metabolites in different cell or tissue types. Analysis of the differentially expressed metabolites can present unique druggable targets to which small molecule therapeutics can be designed.


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