The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 28, 2017
Filed:
Oct. 15, 2014
Applicants:
Zhihao Zhuang, Wilmington, DE (US);
Guorui LI, Baltimore, MD (US);
Inventors:
Zhihao Zhuang, Wilmington, DE (US);
Guorui Li, Baltimore, MD (US);
Assignee:
UNIVERSITY OF DELAWARE, Newark, DE (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07D 317/28 (2006.01); C12Q 1/37 (2006.01); C07K 14/47 (2006.01); C07K 1/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); C07D 317/28 (2013.01); C07K 1/10 (2013.01); C07K 14/47 (2013.01); C07K 2319/50 (2013.01); C07K 2319/95 (2013.01); G01N 2333/95 (2013.01); G01N 2440/36 (2013.01);
Abstract
Deubiquitinating enzyme (DUB) probes are provided that resemble native diubiquitin (diUB) with a similar linkage size and that may contain a Michael acceptor for trapping the DUB active-site cysteine. For example, both K63- and K48-linked diubiquitin probes are generated using a facile chemical ligation method, utilizing the linker compound 3-(2-(bromomethyl)-1,3-dioxolan-2-yl)prop-2-en-1-amine. The diUb probes are capable of labelling DUBs from different families and may be employed to reveal intrinsic linkage specificities of DUBs.