The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 28, 2017
Filed:
Aug. 30, 2012
James O. Mcnamara, Ii, Iowa City, IA (US);
Katie R. Stockdale, Iowa City, IA (US);
Lingyan Huang, Coralville, IA (US);
Alexander R. Horswill, Iowa City, IA (US);
Mark A. Behlke, Coralville, IA (US);
Frank J. Hernandez, Iowa City, IA (US);
James O. McNamara, II, Iowa City, IA (US);
Katie R. Stockdale, Iowa City, IA (US);
Lingyan Huang, Coralville, IA (US);
Alexander R. Horswill, Iowa City, IA (US);
Mark A. Behlke, Coralville, IA (US);
Frank J. Hernandez, Iowa City, IA (US);
University of Iowa Research Foundation, Iowa City, IA (US);
Integrated DNA Technologies, Inc., Coralville, IA (US);
Abstract
The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base.