The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 21, 2017

Filed:

Mar. 13, 2013
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Min Dong, Southborough, MA (US);

Lisheng Peng, Southborough, MA (US);

Pål Erik Gustav Stenmark, Järfälla, SE;

Ronnie Per Arne Berntsson, Åkersberga, SE;

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 39/08 (2006.01); C07K 14/33 (2006.01); C12N 9/52 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/52 (2013.01); C07K 14/33 (2013.01); C12Y 304/24069 (2013.01); A61K 38/00 (2013.01); C07K 2319/55 (2013.01);
Abstract

Disclosed herein areneurotoxin (BoNT) polypeptides with a modified receptor binding domain ofserotype B (B-H), comprising one or more substitution mutations corresponding to substitution mutations in serotype B, strain 1, V1118M; Y1183M; E1191M; E1191I; E1191Q; E1191T; S1199Y; S1199F; S1199L; SI 20 IV; or combinations thereof. Specific combination mutations include E1 191M and S1199L, E1191M and S1199Y, E1191M and S1199F, E1191Q and S1199L, E1191Q and S 1199Y, or E 1191 Q and S 1199F. Other substitution mutations are also disclosed. Isolated modified receptor binding domains, chimeric molecules, pharmaceutical compositions, and methods of using the same are also disclosed.


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