The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 21, 2017
Filed:
Dec. 03, 2014
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan, Hubei, CN;
Jie Ma, Hubei, CN;
Lingbing Zeng, Hubei, CN;
Nan Jiang, Hubei, CN;
Qian Chen, Hubei, CN;
Hui Zhao, Hubei, CN;
Abstract
The present invention discloses a cyprinid herpesvirus II-sensitive brain tissue cell line ofand establishing method and use thereof. The cell line is deposited in China Center for Type Culture Collection under an accession number of CCTCC No: C2013179. The brain tissue cell line ofis in good growth state and sensitive to CyHV-2 that is presently hardly cultured with ordinary fish cell lines. After six passages of CyHV-2 in GiCB cells, viral nucleic acid can still be detected and a cytopathic effect is stable. When ultrathin microscopic sections are prepared from cells having the cytopathic effect, considerable mature CyHV-2 virions and their replication process can be observed in GiCB cells. The construction method of the brain tissue cell line ofof the present invention has high repeatability, scientific and reasonable conditions.